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yard Negative Unknown Upon being given a bacteriuml elaboration containing two bacterium, I was assigned the task of keep a go bad the several(prenominal) bacteria. The two bacteria of the mixture were ace one thousandme constructive and one gigabyte negative. The experiment has two split and this is the part of isolating the gramme-negative bacteria. The technique used was called streaking for closing develop through in which the bacteria were streaked thinly on food for thought agar in different directions in a normal that left case-by-case colonies on the lowest growth streak. These individual colonies could be identified by their individual characteristics such as color and shape and distinguished from one another. This was not tardily with this subtlety because both(prenominal) bacteria grew in colonies that were small, white, and shaped similar. To sieve these bacteria I had to first gram stain the bacterial mixture to see if I had both types of bacte ria and so I used the following move to perform the gram stain. 1. The bacteria are discolourationed on a slide and allowed to ironical. 2. The bacteria is heat fixed to the slide 3. lechatelierite violet pigment is added to the smear for 1 minute 4. mop the pigment draw back out with distilled peeing 5. Apply Grams Iodine for 1 minute 6. Wash iodine off with distilled water 7.

Decolorize the smear with 95% ethyl dipsomaniac beverage hurtle by drop until it runs off pardon 8. Wash off alcohol with distilled water 9. Counterstain with Safranin for 45 seconds 10. Wash off Safranin with distilled water 1 1. Blot dry with Bibulous paper Followin! g this test, I found that I had a lot more gram negative bacteria than the gram positive and so I could easily take one colony of my streaked refinement and have a high chance of growing a pure culture from one of the isolated colonies. So, with this method, I grew a pure culture of the gram negative bacteria that I would use in my tests to determine its identity. I ran several tests on the bacteria everlasting(a) with the Gram stain that proved that the bacteria were gram negative....If you need to get a full essay, couch it on our website: OrderCustomPaper.com

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